Uranium in the structure of Clostridium Perfringens Epsilon Toxin Shows Structural Similarity With the Pore Forming Toxin Aerolysin (pdb 1uyj)

The binding sites of Uranium atom in the structure of Clostridium Perfringens Epsilon Toxin Shows Structural Similarity With the Pore Forming Toxin Aerolysin (pdb code 1uyj). This binding sites where shown with 5.0 Angstroms radius around Uranium atom. The 1uyj structure was solved by A.R.COLE, M.GIBERT, M.POPPOFF, D.S.MOSS, R.W.TITBALL, A.K.BASAK, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 50.0-2.6 Space group P63 a (A) 125.962 b (A) 125.962 c (A) 121.862 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) 23.631 Rfree (%) 29.006 Uranium Binding Sites: Uranium binding site 1 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu137, A: Glu251, conact list: Atom Atom Distance (A) U OE1 A:Glu137 2.71 U OE2 A:Glu137 3.57 U CD A:Glu137 3.47 U CG A:Glu137 4.84 U OE1 A:Glu251 4.65 U OE2 A:Glu251 2.56 U CD A:Glu251 3.59 U CG A:Glu251 3.97 interactive model: Uranium binding site 2 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu70, A: Lys193, conact list: Atom Atom Distance (A) U OE1 A:Glu70 2.54 U OE2 A:Glu70 3.43 U CD A:Glu70 3.36 U CG A:Glu70 4.81 U CE A:Lys193 4.68 interactive model: Uranium binding site 3 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: conact list: Atom Atom Distance (A) interactive model: Uranium binding site 4 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 4 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Glu137, B: Glu251, conact list: Atom Atom Distance (A) U OE1 B:Glu137 2.85 U OE2 B:Glu137 3.12 U CD B:Glu137 3.33 U CG B:Glu137 4.78 U OE1 B:Glu251 4.99 U OE2 B:Glu251 2.88 U CD B:Glu251 3.89 U CG B:Glu251 4.20 interactive model: Uranium binding site 5 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 5 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Glu70, B: Lys193, B: Val195, conact list: Atom Atom Distance (A) U OE1 B:Glu70 2.29 U CB B:Glu70 4.98 U OE2 B:Glu70 2.34 U CD B:Glu70 2.65 U CG B:Glu70 4.19 U CE B:Lys193 4.53 U CG2 B:Val195 4.86 interactive model: Uranium binding site 6 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 6 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Glu232, conact list: Atom Atom Distance (A) U OE1 B:Glu232 2.35 U CB B:Glu232 4.93 U OE2 B:Glu232 2.12 U CD B:Glu232 2.58 U CG B:Glu232 4.12 interactive model: Uranium binding site 7 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 7 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Hoh2012, conact list: Atom Atom Distance (A) U O B:Hoh2012 2.57 interactive model: Uranium binding site 8 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 8 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Lys26, B: Arg28, B: Tyr29, B: Ile55, conact list: Atom Atom Distance (A) U CG B:Lys26 4.90 U CB B:Arg28 4.44 U CD B:Arg28 4.99 U C B:Arg28 4.99 U N B:Tyr29 4.84 U CG2 B:Ile55 4.64 interactive model: Uranium binding site 9 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 9 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: Glu137, C: Glu251, conact list: Atom Atom Distance (A) U OE1 C:Glu137 2.76 U OE2 C:Glu137 3.65 U CD C:Glu137 3.47 U CG C:Glu137 4.79 U OE1 C:Glu251 3.83 U OE2 C:Glu251 2.63 U CD C:Glu251 3.34 U CG C:Glu251 4.22 interactive model: Uranium binding site 10 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 10 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: Tyr182, C: Lys255, C: Glu266, conact list: Atom Atom Distance (A) U OH C:Tyr182 4.35 U CE C:Lys255 2.64 U CD C:Lys255 3.79 U CG C:Lys255 4.49 U OE1 C:Glu266 3.78 U CD C:Glu266 4.56 interactive model: Uranium binding site 11 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 11 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: Glu70, C: Lys193, conact list: Atom Atom Distance (A) U OE1 C:Glu70 2.26 U OE2 C:Glu70 2.95 U CD C:Glu70 2.96 U CG C:Glu70 4.44 U CE C:Lys193 4.50 U NZ C:Lys193 4.51 interactive model: Uranium binding site 12 out of 12 in 1uyj Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 12 of Uranium in the PDB 1uyj. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Uranium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: Arg28, C: Tyr29, C: Lys32, C: Ile55, conact list: Atom Atom Distance (A) U CB C:Arg28 3.91 U CD C:Arg28 4.31 U C C:Arg28 4.87 U CG C:Arg28 4.70 U N C:Tyr29 4.59 U CE C:Lys32 4.94 U CG2 C:Ile55 4.28 interactive model: